CSE Doctoral Student Seminar: Mingquan Yuan and Xiaoxin Liu

Sep 23, 2016
12:30 p.m.
2:00 p.m.
Lopata Hall, Room 101

​​​Mingquan Yuan
Advisor: Shantanu Chakrabartty

'Self-powered Wireless Biosensing Based on Integration of Paper-based Microfluidics with Self-assembling RFID Antennas"

A wireless, self-powered, affinity-based biosensor based on the integration of paper-based microfluidics with our previously reported method for self-assembling radio-frequency (RF) antennas will be introduced. At the core of the proposed approach is a silver-enhancement technique that grows portions of an RF antenna in regions where target antigens hybridize with target specific affinity probes. The hybridization regions are defined by a network of nitrocellulose based microfluidic channels which implement a self-powered approach to sample the reagent and control its flow and mixing. The integration substrate for the biosensor has been constructed using polyethylene and the patterning of the antenna on the substrate has been achieved using a low-cost ink-jet printing technique. The substrate has been integrated with passive radio-frequency identification (RFID) tags to demonstrate that the resulting sensor-tag can be used for continuous monitoring in a food supply-chain where direct measurement of analytes is typically considered to be impractical. We validate the proof-of-concept operation of the proposed sensor-tag using IgG as a model analyte and using a 915 MHz Ultra-high-frequency (UHF) RFID tagging technology. ​A wireless, self-powered, affinity-based biosensor based on the integration of paper-based microfluidics with our previously reported method for self-assembling radio-frequency (RF) antennas will be introduced. At the core of the proposed approach is a silver-enhancement technique that grows portions of an RF antenna in regions where target antigens hybridize with target specific affinity probes. The hybridization regions are defined by a network of nitrocellulose based microfluidic channels which implement a self-powered approach to sample the reagent and control its flow and mixing. The integration substrate for the biosensor has been constructed using polyethylene and the patterning of the antenna on the substrate has been achieved using a low-cost ink-jet printing technique. The substrate has been integrated with passive radio-frequency identification (RFID) tags to demonstrate that the resulting sensor-tag can be used for continuous monitoring in a food supply-chain where direct measurement of analytes is typically considered to be impractical. We validate the proof-of-concept operation of the proposed sensor-tag using IgG as a model analyte and using a 915 MHz Ultra-high-frequency (UHF) RFID tagging technology.

Xiaoxin Liu
Advisor: Weixiong Zhang

"Circular RNAs in the AD brain"

Circular RNA (circRNA) is a novel discovered type of RNA, which forms a closed continuous loop instead of linear structure like messenger RNA. It is widely detected in animals and plants, especially enriched in human neural cells. We​ developed a novel circRNA identification method to search for all possible back fusion points (BFP) within the RNA-seq reads. By applying this method to 80 samples from human AD (Alzheimer's disease) and normal brains, we detected 112 circRNAs and 7 are differentially expressed by analyzing with RankSum. Five of these aberrantly expressed circRNAs have host genes related with neural disorders. The results from this genome-wide investigation of circRNAs in the AD brain substantiated that circRNAs are enriched and some are differentially expressed, indicating that circRNAs may play a role in the AD brain.