Mussel foot protein is sticky; engineering researchers can make it stickier

Mussels stick to rocks on the seafloor, to aquatic plants, and — to the consternation of boaters — they can hitch rides fastened to seafaring vessels no matter their composition: metals, rubber, glass, wood and more.

But today’s bothersome bivalves may help researchers to develop a new means to mend a wound.

Scientists at the McKelvey School of Engineering at Washington University in St. Louis have replicated the mussel’s sticky protein and are now working to improve it for everyday use. The strong, underwater adhesive could have many applications, it could even help to fix the very boats now vexed by the mollusks.

“Most synthetic glues do not work on wet surfaces, but mussel foot proteins (Mfp) can adhere to surfaces even underwater,” said Fuzhong Zhang, associate professor of energy, environmental & chemical engineering. “This unique property makes Mfp suitable for many applications, from underwater repair to biomedical glues. With further engineering, an Mfp glue could be used to heal wounds, or maybe even to replace sutures.”

Zhang has received a $502,034 grant from the Office of Naval Research to improve on their initial success, microbially produced underwater adhesives that are stronger than those created naturally by mussels.

Research in the Zhang lab focus on engineering microbes to produce renewable materials and chemicals with desired properties, a field called synthetic biology. An example of his team’s work includes engineering bacteria that produced synthetic spider silk as strong and tough as natural spider silk fibers. For adhesive proteins, in research published in late 2018, his team demonstrated that synthetic proteins made by engineered bacteria can be even more adhesive than natural Mfps when used underwater.

This image shows the strength of a new, synthetic adhesive developed by Zhang. Created by engineered microbes and modeled after the substance that mussels use to stick to submerged surfaces, this adhesive will also work under water. (Graphic: Zhang lab)

 The power of synthetic biology is that researchers can fine tune the property of protein materials by manipulating their genetic codes. “We can change one parameter each time,” Zhang said. For instance, the length of a chain of Mfp5. “If with an increase of one parameter, we see adhesion becomes better and better, then we can say, ‘OK, this parameter is able to increase adhesion,’” he said.

It turns out, that is exactly what the team has found: when the Mfp protein chain was made longer, its underwater adhesion was stronger than that with natural chain length.

This approach could allow them to explore many unknown mechanisms that control underwater adhesion, parameter-by-parameter. “Understanding the mechanism by synthesis has the potential to lead to additional enhancements in adhesion, or improve other glue properties.” Zhang said. “In general, we’re trying to understand the mechanism using synthetic biology by producing and testing the materials.”

The McKelvey School of Engineering at Washington University in St. Louis promotes independent inquiry and education with an emphasis on scientific excellence, innovation and collaboration without boundaries. McKelvey Engineering has top-ranked research and graduate programs across departments, particularly in biomedical engineering, environmental engineering and computing, and has one of the most selective undergraduate programs in the country. With 140 full-time faculty, 1,387 undergraduate students, 1,448 graduate students and 21,000 living alumni, we are working to solve some of society’s greatest challenges; to prepare students to become leaders and innovate throughout their careers; and to be a catalyst of economic development for the St. Louis region and beyond.

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