Researchers uncover how cells interact with supporting proteins to heal wounds

A Washington University in St. Louis team provides new insight into wound healing, fibrosis or cancer metastasis

Beth Miller 
Fibroblast morphology in 3D with no actomyosin contractility.
Fibroblast morphology in 3D with no actomyosin contractility.

When we get a wound on our skin, the cells in our bodies quickly mobilize to repair it. While it has been known how cells heal wounds and how scars form, a team led by researchers from Washington University in St. Louis has determined for the first time how the process begins, which may provide new insight into wound healing, fibrosis or cancer metastasis.

The team, led by Delaram Shakiba, a postdoctoral fellow from the NSF Science and Technology Center for Engineering Mechanobiology (CEMB) in the McKelvey School of Engineering, discovered the way fibroblasts, or common cells in connective tissue, interact with the extracellular matrix, which provides structural support as well as biochemical and biomechanical cues to cells. The team uncovered a recursive process that goes on between the cells and their environment as well as structures in the cells that were previously unknown.

Results of the research are published in ACS Nano July 28, 2020. Senior authors on the paper are Guy Genin, the Harold and Kathleen Faught Professor of Mechanical Engineering in the McKelvey School of Engineering, and Elliot Elson, emeritus professor of biochemistry and molecular biophysics at the School of Medicine.

"Clinical efforts to prevent the progression of fibrocontractile diseases, such as scarring and fibrosis ,have been largely unsuccessful, in part because the mechanisms that cells use to interact with the protein fibers around them are unclear," Shakiba said. "We found that fibroblasts use completely different mechanisms in the early — and I think the most treatable — stages of these interactions, and that their responses to drugs can therefore be the opposite of what they would be in the later stages."

Genin, who is the co-director of the CEMB, said the process has stymied mechanobiology researchers for some time.

"Researchers in the field of mechanobiology thought that cells pulled in collagen from the extracellular matrix by reaching out with long protrusions, grabbing it and pulling it back," Genin said. "We discovered that this wasn't the case. A cell has to push its way out through collagen first, then instead of grabbing on, it essentially shoots tiny hairs, or filopodia, out of the sides of its arms, pulls in collagen that way, then retracts."

Now that they understand this process, Genin said, they can control the shape that a cell takes.

"With our colleagues at CEMB at the University of Pennsylvania, we were able to validate some mathematical models to go through the engineering process, and we now have the basic rules that cells follow," he said. "We can now begin to design specific stimuli to direct a cell to behave in a certain way in building a tissue-engineered structure."

The researchers learned they could control the cell shape in two ways: First, by controlling the boundaries around it, and second, by inhibiting or upregulating particular proteins involved in the remodeling of the collagen.

Fibroblasts pull the edges of a wound together, causing it to contract or close up. Collagen in the cells then remodels the extracellular matrix to fully close the wound. This is where mechanobiology comes into play.

"There's a balance between tension and compression inside a cell that is newly exposed to fibrous proteins," Genin said. "There is tension in actin cables, and by playing with that balance, we can make these protrusions grow extremely long," Genin said. "We can stop the remodeling from occurring, or we can increase it."

The team used a 3D-mapping technique — the first time it has been applied to collagen — along with a computational model to calculate the 3D strain and stress fields created by the protrusions from the cells. As cells accumulated collagen, tension-driven remodeling and alignment of collagen fibers led to the formation of collagen tracts. This requires cooperative interactions among cells, through which cells can interact mechanically.

"New methods of microscopy, tissue engineering and biomechanical modeling greatly enhance our understanding the mechanisms by which cells modify and repair the tissues they populate," Elson said. "Fibrous cellular structures generate and guide forces that compress and reorient their extracellular fibrous environment. This raises new questions about the molecular mechanisms of these functions and how cells regulate the forces they exert and how they govern the extent of matrix deformation. "

"Wound healing is a great example of how these processes are important in a physiologic way," Genin said. "We'll be able to come up with insight in how to train cells not to excessively compact the collagen around them."


Shakiba D, Alisafaei F, Savadipour A, Rowe RA, Liu Z, Pryse K, Shenoy V, Elson EL, Genin GM. The Balance Between Actomyosin Contractility and Microtubule Polymerization Regulates Hierarchical Protrusions that Govern Efficient Fibroblast-Collagen Interactions. ACS Nano, July 28, 2020. DOI: 10.1021/acsnano.9b09941

Funding for this research was provided by the National Institutes of Health (5-T32-HL07081-38); the National Science Foundation Science and Technology Center for Engineering Mechanobiology (CMMI-154857); National Cancer Institute (U01CA202177, U54CA193417 and R01CA232256), and National Institute of Biomedical Imaging and Bioengineering (R01EB017753).


The McKelvey School of Engineering at Washington University in St. Louis promotes independent inquiry and education with an emphasis on scientific excellence, innovation and collaboration without boundaries. McKelvey Engineering has top-ranked research and graduate programs across departments, particularly in biomedical engineering, environmental engineering and computing, and has one of the most selective undergraduate programs in the country. With 165 full-time faculty, 1,420 undergraduate students, 1,614 graduate students and 21,000 living alumni, we are working to solve some of society’s greatest challenges; to prepare students to become leaders and innovate throughout their careers; and to be a catalyst of economic development for the St. Louis region and beyond.

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